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Andrija Stampar School of Public Health
Zagreb University Medical School
Rockefellerova 4
HR-10000 Zagreb, Croatia
P: +385.1.4590100 / 142
F: +385.1.4684212
Accepted 22 December 2005
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A condition called "corporal fibrosis" -- where the tubes of penile "spongy tissue" that maintain erection are gradually replaced by inactive, fibrous scar tissue -- remains largely untreatable. The disorder occurs when the penis' sensitive spongy-tissue cells don't get the oxygen they need to survive, usually because of a chronic reduction in blood flow.
Mon, Apr 27 2009 NEW YORK (Reuters Health) - Two disorders that seem completely unrelated except that each is the focus of massive drug company ad campaigns may actually have something in common: Older men who suffer from restless legs syndrome at night are almost twice as likely to have erectile dysfunction as those without restless legs, researchers report.
Cell purification. CD11b+ purification was performed with mouse CD11b MicroBeads (Miltenyi Biotec). The positive and negative fractions were sorted with the LS columns according to the manufacturer's instructions. CD11c+ cells were positively selected from splenocytes of tumor-bearing mice using mouse CD11c MicroBeads (Miltenyi Biotec). CD4+ or CD8+ T cells were negatively selected, using the CD4+ or CD8+ T cell isolation kit (Miltenyi Biotec), from CD11b-depleted splenocytes. CD14 depletion of human PBMCs was obtained by adding PE-labeled, anti–human CD14 antibody (BD Biosciences). The CD14-negative population was collected using a cell sorter (FACSVantage SE; BD Biosciences).
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